质粒名称: | pFUGW-H1 empty vector |
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出品公司: | 爱迪基因 |
目录编号: | 25870 |
存储实验室: | Sally Temple |
载体骨架基本信息 |
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载体名称: | FUGW-H1 |
载体出品公司: | Ivanova, NB |
载体抗性: | 氨苄霉素 |
完整质粒大小: | 10130 bp |
载体类型: | 哺乳细胞表达,慢病毒载体 |
筛选标记: | 博莱霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | None |
启动子: | H1 |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | see below(未破坏) |
3' 克隆位点: | see below(未破坏) |
5' 测序引物 | H1 (5'-tcgctatgtgttctgggaaa-3') |
3' 测序引物 | cagtgcaggggaaagaatagtagac |
文献引用方法 |
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材料和方法部分: | pFUGW-H1 empty vector was a gift from Sally Temple (爱迪基因 plasmid # 25870) |
参考文献部分: | shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S. Cell Stem Cell. 2007 Jun 7. 1(1):87-99. 10.1016/j.stem.2007.04.001 PubMed 18371338 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pFUGW-H1 empty vector 10130 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION 3rd generation lentiviral vector. ACCESSION . VERSION . KEYWORDS pFUGW-H1 empty vector SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10130) AUTHORS Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S TITLE shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. JOURNAL Cell Stem Cell. 2007 Jun 7. 1(1):87-99. PUBMED 18371338 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pFUGW-H1 empty vector| FEATURES Location/Qualifiers source 1..10130 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 417..538 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(454..473) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 508..527 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(587..603) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(587..603) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(600..622) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 611..627 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(635..665) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 680..701 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(818..835) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(989..1577) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1069..1088) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1748..2608) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2371..2390 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2609..2713) /gene="bla" /label=AmpR promoter primer_bind complement(2788..2807) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 2979..3358 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3360..3558 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3513..3533 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 3576..3756 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 3803..3928 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 4421..4654 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 5181..5298 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter complement(5402..5616) /label=H1 promoter /note="human H1 RNA promoter" primer_bind complement(5462..5481) /label=H1 /note="Human H1 promoter, forward primer" promoter 5645..6044 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 6693..6712 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 6819..6828 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(6870..6891) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7131..7150) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7479..7500 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7572..8160 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7625..7645) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8043..8054) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(8163..8179) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8164..8180) /label=pBluescriptKS /note="For pBluescript vector" LTR 8685..8865 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8882..8899) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8888..9112 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9158..9586 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9245..9264) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9455..9476 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9595..9615) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9600..9929 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9780..9915 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9842..9861 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9977..10024 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 10043..287 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" ORIGIN 1 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 61 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 121 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 181 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 241 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag 301 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 361 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 421 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 481 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 541 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 601 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 661 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 721 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 781 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 1021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 1081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 1141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 1201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 1261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 1321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 1381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 1441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 1501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 1561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 1621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 1681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 1741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 1801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 1861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 1921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 1981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 2041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 2101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 2161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 2221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 2281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 2341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 2401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 2461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 2521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 2581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 2641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 2701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 2761 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2821 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 2881 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt 2941 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta 3001 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 3061 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 3121 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 3181 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 3241 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 3301 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 3361 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc 3421 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt 3481 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg 3541 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct 3601 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 3661 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 3721 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa 3781 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac 3841 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta 3901 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg 3961 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg 4021 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg 4081 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag 4141 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga 4201 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca 4261 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg 4321 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa 4381 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt 4441 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca 4501 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc 4561 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg 4621 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 4681 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga 4741 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa 4801 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg 4861 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata 4921 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac 4981 tttgtatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc 5041 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca 5101 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa 5161 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 5221 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 5281 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt 5341 aagaaaggat ccgaaagaat tcgcccttag atctctagag gagctttctt cccgggaaag 5401 agtggtctca tacagaactt ataagattcc caaatccaaa gacatttcac gtttatggtg 5461 atttcccaga acacatagcg acatgcaaat attgcagggc gccactcccc tgtccctcac 5521 agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc cgcctagtga cactgggccc 5581 gcgattcctt ggagcgggtt gatgacgtca gcgttcttaa ttaacccgtg tcggctccag 5641 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc 5701 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg 5761 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt 5821 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc 5881 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc 5941 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt 6001 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg 6061 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga 6121 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg 6181 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct 6241 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg 6301 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga 6361 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg 6421 cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg 6481 tgtcgtgacg tnncccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg 6541 tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct 6601 gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg 6661 gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc 6721 ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg 6781 gtcaatatgt aattttcagt gttagactag taccggtcgc caccatggtg agcaagggcg 6841 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 6901 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 6961 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 7021 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 7081 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 7141 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 7201 tgaagggcat cgacttcaag gtaggacggc aacatcctgg ggcacaagct ggagtacaac 7261 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 7321 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 7381 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 7441 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 7501 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcaa ttcgatatca 7561 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 7621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 7681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 7741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 7801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 7861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 7921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 7981 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 8041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 8101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 8161 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag 8221 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc 8281 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 8341 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata 8401 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac 8461 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 8521 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga 8581 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc 8641 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag 8701 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 8761 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 8821 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagaaac ccgctgatca 8881 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 8941 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 9001 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 9061 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 9121 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 9181 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 9241 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 9301 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 9361 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 9421 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 9481 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 9541 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 9601 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 9661 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 9721 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 9781 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 9841 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 9901 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 9961 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 10021 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 10081 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct //
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